Coding

Part:BBa_K525010:Design

Designed by: Manuel Wittchen   Group: iGEM11_Bielefeld-Germany   (2011-10-25)


Fusion Protein of S-Layer SbpA and LigA with His-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 221
    Illegal BglII site found at 4611
    Illegal XhoI site found at 1996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 493
    Illegal BsaI.rc site found at 622
    Illegal SapI.rc site found at 4825


Design Notes

Assembled with Freiburg Standard Assembly (RFC 25)


Source

SbpA was synthesized, LigA is the native ligase of E. coli.

References